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Hello,
I've made numerous minor edits to this page to make it conform with Wikipedia's style, and I also added a "cleanup" template message at the top to encourage others to continue improving the page. But since this a low priority page according to WikiProject Molecular and Cellular Biology and WikiProject Computational Biology and therefore doesn't receive much attention, I took it upon myself to rewrite the page to remove promotional and overly technical language. However, I have a conflict of interest and cannot ethically make edits myself. Therefore, I kindly request that another replace the content on the page with the content below. You'll notice that besides removing inappropriate language, I did not add any new information, except citations and crosslinks where they were lacking.
Here is my full rewrite:
ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility.[1] In 2013, the technique was first described as an alternative advanced method for MNase-seq (sequencing of micrococcal nuclease sensitive sites), FAIRE-Seq and DNase-Seq.[1] ATAC-seq is a faster and more sensitive analysis of the epigenome than DNase-seq or MNase-seq.[2][3][4]
ATAC-seq identifies accessible DNA regions by probing open chromatin with hyperactive mutant Tn5 Transposase that inserts sequencing adapters into open regions of the genome. [2][5] While naturally occurring transposases have a low level of activity, ATAC-seq employs the mutated hyperactive transposase.[6] In a process called "tagmentation,"Tn5 transposase cleaves and tags double-stranded DNA with sequencing adaptors.[7] The tagged DNA fragments are then purified, PCR-amplified, and sequenced using next-generation sequencing.[7] Sequencing reads can then be used to infer regions of increased accessibility as well as to map regions of transcription factor binding sites and nucleosome positions.[2] The number of reads for a region correlate with how open that chromatin is, at single nucleotide resolution.[2] ATAC-seq requires no sonication or phenol-chloroform extraction like FAIRE-seq;[8] no antibodies like ChIP-seq;[9] and no sensitive enzymatic digestion like MNase-seq or DNase-seq.[10] ATAC-seq preparation can be completed in under three hours.[11]
Modifications to the ATAC-seq protocol have been made to accommodate single-cell analysis. Microfluidics can be used to separate single nuclei and perform ATAC-seq reactions individually.[11] With this approach, single cells are captured by either a microfluidic device or a liquid deposition system before tagmentation.[11] [12] An alternative technique that does not require single cell isolation is combinatorial cellular indexing. This technique uses barcoding to measure chromatin accessibility in thousands of individual cells; it can generate epigenomic profiles from 10,000-100,000 cells per experiment.[13] But combinatorial cellular indexing requires additional, custom-engineered equipment or a large quantity of custom, modified Tn5. [14]
Thank you, cglife.bmarcus (talk) 1:06, 5 September 2019 (UTC)
References
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This edit request by an editor with a conflict of interest has now been answered. |
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, as the references listed were their own publications covering a specialized area of information and published in editions (as are textbooks), rather than typical journals which publish on a regular schedule and which usually cover discordant topics (linked by a category) under sequential volumes and issues.Regards, Spintendo 10:37, 12 September 2019 (UTC)
This article was the subject of a Wiki Education Foundation-supported course assignment, between 29 August 2022 and 15 December 2022. Further details are available on the course page. Student editor(s): Cornonthekaba (article contribs).
— Assignment last updated by Cornonthekaba (talk) 17:17, 5 October 2022 (UTC)