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Perhaps a section about how DNA is synthesized in the laboratory?
DNA can be synthesized in vitro in the laboratory by thermocyclic or isothermal reactions, these methods are called DNA amplification technologies, mainly include:
[1] Thermocycling reactions
(1) PCR: Polymerase Chain Reaction, Science,1985
(2) LCR: Ligase Chain Reaction, PNAS,1991
(3) PEAR: Polymerase-Endonuclease Amplification Reaction, PLoS One, 2010
[2] Isothermal reactions
(1) SDA: Strand Displacement Amplification, PNAS,1992
(2) RCA: Rolling circle amplification, Nature Genetics,1998
(3) LAMP: loop-mediated isothermal amplification, NAR, 2000
(4) HDA: helicase-dependent amplification, EMBO reports, 2004
(5) EXPAR: Exponential amplification reaction, PNAS, 2003
--Xiaolong7292 (talk) 01:21, 24 August 2011 (UTC)
I have deleted eukaryotic and prokaryotic replication and put it on its separate pages. There is too many specific differences in theses two topics and I will try to fix the entire page, and organize it better. 03/06/07
shaon==
I think there should also be another section discuss the similiarties between eukaryotic and archeal (and archeal - prokaryotic) DNA replication. This is important because it deals with evolution and the relatedness between eukaryotes and archea.see it,,
Shouldn't "ozaki fragments" in the picture (png) be "okazaki fragments"?
rolling circle rep include also
For those of you who know a little more about the licencing factors, how about an explanation of why gene shearing is an accepted technology - do we really know what control mechanisms are involved here ? Or is the inherent capacity of the cell to allow for our meddling, increasingly a source of wonder?
I looked at this page while doing some homework for my high school biotechnology class and found that it was missing a lot of key information. For example:
-DNA-B first marks the initiation site for Rep (aka Helicase) to begin "cutting" the DNA -DNA Gyrase relaxes the supercoiling so that the section of DNA that needs to be replicated is stretched out -topoisomorase (not sure on spelling) breaks covelant bonds (not sure why, I believe so that Rep has a place to start cutting the strands)
Also, the DNA polymerase referred to in the picture is DNA Polymerase III, and DNA polymerase I is involved in removing the segments between the Okazaki fragments (because they were created by RNA Primase and use RNA nucleotides).
I would just edit the article, but this is from notes I took from my teachers lecture and I'm not sure whether or not to submit...
Topoisomerase and DNA Gyrase are the same enzyme, it just has action in both supercoiling and uncoiling, the fluoroquinolone class of drugs work here actually. Additionally the picture is an extremely simplified representation of DNA synthesis, rather than edit it I would look for a more complete picture that has Pols 1-3 and a description of what they do and in which direction they do it. Most Pols have action in both the 3-5 and 5-3 directions. Pol 1 removes the RNA primers and repairs the region with the last few bases between fragments, ligase is still required for the final 'stitch' between the fragments.
Etherealmuse 8:05, 17 Feb 2005 (UTC)
This page also fails to address the obvious question of how the enzymes know how to complete these complex tasks. As it is, we appear to have an obscurium per obscurius, whereby the propensities of celled creatures are explained by a code, but the actions of enzymes remains a mystery. Are enzymes also driven by a code or are they automatically tripped, like thermostats? It is implied by the terminology used if nothing else that decisions are being made by these enzymes. How do enzymes make decisions? If there is a Wiki article that begins to explain this, there should be a link. Neither the enzyme nor the enzyme kinetics article does so.81.164.252.74 00:57, 6 August 2006 (UTC)
The information about the speed of replication in humans had an error that I cleaned up quickly. It said something to the extent that the an average human chromosome had 6 billion base pairs. This is wrong, the entirety of the human genome has ~6 billion base pairs, which are themselves arranged in 46 linear chromosomes. Average chromosome length is not a particularly good reference as it varries considerably (spanning several orders of magnitude). I cleaned it up to reflect this fact.
-Mr. Knuffke
If anyone knows it would probably be worth putting in the article. —Preceding unsigned comment added by 80.42.104.21 (talk • contribs) 22:13, 7 April 2006
DNA replication proceed in the 5' to 3' direction because the substrates for DNA replication, like RNA polymerization, are 5' nucleoside triphosphates. In DNA replication the 2' deoxynucleoside triphosphates elongate nacent chains in a template-directed fashon by cleavage of pyrophosphate (the terminal two of three phosphate groups) and transesterification of the remaining phosphate to the free 3- hydroxyl group of the growing chain. This increases the chain length by one nucleoside, generates pyrophosphate, and reconstitutes another 3' hydroxly group for continued chain elongation. —Preceding unsigned comment added by 71.128.53.151 (talk • contribs) 15:09, 1 May 2006
Also: The asymmetery of the promoter sequence —Preceding unsigned comment added by 83.100.173.204 (talk • contribs) 21:24, 16 October 2006
The reverse sequence 3- to 5- polymerization does occur in nature but would require a different mechanism to dominate the replication process. Prof. Eric Lander (MIT) postulated an explanation of a possible reason natural selection preferred the current order. The reverse process would use contributing molecuels that are more favored for other reactions and thus less available the process. Watch http://ocw.mit.edu/courses/biology/7-012-introduction-to-biology-fall-2004/video-lectures/
You can not watch more than 10 min of Erics lectures without seeing something that should go in wikipedia. We might be getting a little too technical for wikipedia?? I wouldn't want anyone brain to explode.—Preceding unsigned comment added by Scottprovost (talk • contribs) 20:35, 4 August 2007
In my opinion there should be to separate articles for DNA replication in prokaryotes and DNA replication in eukaryotes. At least it should be made clear if prokaryotic DNA replication or eukaryotic is described. I think I know these two processes quite well, but people who read this to learn about DNA transcription will be unnecessarily confused by unclear parts in this article.
Additionally I think the different eukaryotic polymerases should be mentioned. They are very essential! I suggest copying some of the information from the wikipedia article DNA polymerases.
--Happy Puppy 01:38, 2 August 2006 (UTC)
I'm a Year 12 biology student, and I had heaps of trouble understanding much of this page. --218.215.130.252 10:36, 20 August 2006 (UTC)
I am suggesting that we add a picture found on the main DNA page, but not found here. The pic to be added to theis page is found here: http://en.wikipedia.org/wiki/Image:Dna-split.png
I do not know how to add pics, so please can either someone tell me, or post a link to where i can learn how to myself on my talk page.
Just a quick note - I agree with 218.215.130.252, It is a bit complicated, so a SIMPLE summary section would be nice. I am doing DNA as part of my GCSEs in the UK.
Thanks
Stwalkerster 21:23, 16 September 2006 (UTC)
I may have failed the DNA unit in Biology, but when I was working on an assignment I got confused. Is this DNA replication or protein synthesis?
--Falconus|Talk 00:43, 10 October 2006 (UTC)
I was reading the DNA replication, and unable to find information pertaining DNA replication's bidirectionality. I think it is very important to have a section to explain the DNA's bidirectionality replication of a chromosome, as well as a contradictary example of unidirectionality. —The preceding unsigned comment was added by Wolingfeng (talk • contribs) 22:25, 6 December 2006 (UTC).
I saw that people were saying how unclear the article was, so I made some changes (but am not done yet). Could someone, preferably someone who is not a molecular biologist or a biochemist :), read few of my changes, so I know if it is understandable to a more general public?
I will make further changes/addition in upcoming days.
also, if someone has more/better ideas, change stuff around!
thanks, Svetlana Miljkovic 13:12, 20 December 2006 (UTC)
http://www.wehi.edu.au/education/wehi-tv/dna/replication.html This little animation from different angles should be helpful for those interested how DNA replication works. I think we should add this link since Drew Berry made an understandable animation here...--Artman40 10:38, 30 December 2006 (UTC)
The former content of this article appears to be at DNA replicate. I think it makes most sense to merge it back into this article here. I'm not sure why it was separated out in the first place. - tameeria 19:24, 8 March 2007 (UTC)
I support the merge, Also DNA replicate seems to be the stronger article. It also seems to be 5 years newer then DNA replication Leafyplant 19:32, 8 March 2007 (UTC)
Other candidates for merges include replication fork, lagging strand, and leading strand. All these are stubs right now and topics that should be part of this article. (As far as DNA replicate goes, it appears that article's content was copied from a former version of this article at the beginning of February this year, after which this article was heavily edited away from its former content so that we have two completely different articles on the same topic now.) - tameeria 19:34, 8 March 2007 (UTC)
As a student, I find that having separate entries is extremely useful. Maristoddard 17:07, 17 July 2007 (UTC)
Since there's been no movement on this, I'll remove the porposed merge tag. I think the topics are probably better as separate entities to simplify this page. — Scientizzle 22:48, 8 October 2007 (UTC)
The sections on prokaryotic DNA replication and eukaryotic DNA replication also have their own pages. Therefore, these sections could be reduced to a brief summary here. Alternatively, their pages could redirect here, so that the content is not duplicated. I'm not sure what would be the best approach, but given the amount of details, I think it might be best to keep this article general and have the specifics for pro- and eukaryotes in those other articles? - tameeria 19:43, 8 March 2007 (UTC)
It still needs a lot of work, and a lot of new sections need to be added to it. At the moment, I study microbiology at the university of guelph. But a lot of sections still need to be edited for more "completeness" because I either pulled them right from the wikipedia page (like d-loop, lagging/leading/replication fork) and need to be cleaned up. sorry for my bad grammer, ive been up all night doing final edits on my h2ax paper so im just a little grammered out! but I think the next major section should be archea and replication in bacterial and eukaryotic viruses. As for the way eukaryotic and prokaryotic replication is structured..i think its fine as it is now (maybe a section pointing to the mechanism of elongation and a blurb explaining that) and then the pages that you started with it could be further detailed later on...i think we also need some way to talk about checkpoints in S-phase in the eukaryotic cell cycle with regards to stalled replication forks and double strand breaks and other forms of DNA damage that can occur in DNA replication..nothing huge, but something pointing to DNA repair (And hopefully if i get a good grade on my paper, ill turn it into a wikipedia page!)..anyways, enough of my blabbing - Joe
Based on previous comments, it appears the redirect of DNA synthesis to this article might be ambiguous. Besides cellular DNA replication, there is also enzymatic in vitro DNA replication (Polymerase chain reaction) and chemical DNA synthesis (oligonucleotide synthesis). Should DNA synthesis be disambiguated? Also, information on chemical DNA synthesis was requested, but I think it's beyond the scope of this article as it is not exactly replication, but de-novo synthesis via chemistry. What would be a good title for a page for that topic? Should it be at DNA synthesis, or should that be purely a disambigation page with the other maybe at Oligonucleotide synthesis? - tameeria 19:49, 8 March 2007 (UTC)
This page seems to be suffering from some major issues. In my opinion, it would be best to make the distinction between prokaryotic and eukaryotic DNA replication more obvious, preferably by splitting everything except the introduction in a prokaryotic and a eukaryotic section. Also, it doesn't have in-line references. Since those are preferred for good article status, I will add those in my edits. If the previous sources could be converted to in-line references it would be great. Sakkura 18:18, 13 July 2007 (UTC)
I feel these eubacteria / eukaryotes / archaea sections are very heavy on naming of specific enzymes rather than focusing on general mechanisms. I think it would be nice to reorganize this article with more general mechanisms of DNA replication rather than throwing case studies at the reader. I was looking at chapter 15 in DNA Replication 2nd ed (Kornberg & Baker) which lists some basic rules of replication:
Rather than throw three different cases at the reader, we could talk about these common features. If the differences between eukaryote and eubacteria and archaea can be boiled down into a paragraph, this would be ideal.
It's not that the current material is wrong, but it's way, way too technical. I've got biology background and my eyes just glaze over when I read: "Activation of the complex occurs in S-phase and requires Cdk2-Cyclin E and Ddk. The activation process begins with the addition of Mcm10 to the pre-RC, which displaces Cdt1. Following this, Ddk phosphorylates Mcm3-7, which activates the helicase."
I feel bad throwing material away, but I feel like the page needs a rewrite that focuses on common mechanisms rather than separately describing a couple of very detailed biochemical cases. I'd like to start working on it myself, please let me know if I shouldn't spend time on it. Madeleine ✉ ✍ 20:25, 1 August 2007 (UTC)
Hey I don't know who is writing this article, but I am in 11th grade and I didn't understand much from this, remember that a lot of people check this page and has to be understandable for everyone!--189.135.137.8 20:54, 6 October 2007 (UTC)
I disagree also. I'm a 3rd year Med Student who just couldn't grasp this concept last year in class. HOWEVER, after reading this article, utilizing the FANTASTIC visual aides, plus visiting the word links that I didn't understand... It pulled it all together for me. The lightbulb finally turned on for me. I am so grateful, thank you. USMLE Step 1 here I come! — Preceding unsigned comment added by 12.235.208.2 (talk) 15:17, 13 November 2011 (UTC)
I've finally put together a draft of a new version of this article that focuses more on common mechanisms. I've thrown away a lot of the protein naming. It would be wonderful if anyone interested could take a look at the article, make corrections, suggestions and/or mark it up with ((fact)) and ((what)) tags (I tend to write things in one fell swoop and it's hard to know which sentences should get references placed on them so it'd be nice if someone else marked which points should be referenced). Tell me what you think and maybe I can copy it over here and replace the current article. Madeleine ✉ ✍ 19:16, 3 March 2008 (UTC)
In the wikipedia article it says: "DNA polymerases are generally extremely accurate, making less than one error for every million nucleotides added."
But according to one of the sources cited:
"As discussed at the beginning of this chapter, the fidelity of copying DNA during replication is such that only about 1 mistake is made for every 10^9 nucleotides copied."
That makes it less than an error in 1 billion nucleotides added, not 1 million. If people agree can we change it according to the source? [5] —Preceding unsigned comment added by Medfreak (talk • contribs) 13:08, 22 August 2008 (UTC)
I vaguely remember learning about a name for the active site where replication was occuring. Isn't there a name for this region of splitting and replicating? Isn't there a sub-organelle or micro-organelle responsible for this? Stephen Charles Thompson (talk) 21:12, 24 April 2009 (UTC)
It was previously mentioned in this discussion, but now that the same discussion in that other article Talk:Replication fork, it's worth to bring up again. It says almost exactly the same as the section DNA replication#The replication fork and could conveniently be redirected directly to that section. Thus, there's no use reading Replication fork after reading this article, and starting with reading Replication fork without getting it in its context is also inefficient. Mikael Häggström (talk) 18:54, 21 May 2009 (UTC)
After reading both articles, the similarity is quite apparent; the merging of these two pages makes sense in terms of completeness. However, there was an earlier discussion about the simplicity of this article. As an 11th grader I can easily understand the plight of several High School Biology students, and for their sake, I feel that these articles should remain separate. If someone needs to go deeper, (as I did) it is easy enough to follow the link and skim the repetitious material. --Adam zappul (talk) 03:54, 6 June 2010 (UTC)
I think that the merging does make a lot of sense, being a "High School Biology student," as you put it. The replication fork is in the very first picture in the article, and is the beginning of DNA replication, so I would say it would make more sense to merge the two. --~tajt 16:41, 18 November 2010 (UTC)
Sorry for not having an account, but I figured my voice needed to be heard. I'm currently studying for midterms in my high school and this is on the biology test and I really need this. Please keep and don't merge :) 76.250.195.66 (talk) 16:12, 18 January 2011 (UTC)
We have a few unclear sentences here
Debresser (talk) 14:38, 4 September 2009 (UTC)
In the section under Origins of Replication, there is a reference to Okazaki fragments as follows: "Once strands are separated, RNA primers are created on the template strands. More specifically, the leading strand receives one RNA primer per active origin of replication while the lagging strand receives several; these several fragments of RNA primers found on the lagging strand of DNA are called Okazaki fragments, named after their discoverer."
It seems to me that it would be beneficial to hyperlink the term "Okazaki fragments" to the Wiki article of the same name....also possibly pluralizing the word "discoverer", since in the Okazaki fragment Wiki article two Okazakis are credited with their discovery. Just a couple of thoughts after reading the first few paragraphs of the article. 66.205.144.176 (talk) 11:12, 6 September 2010 (UTC)introspectacle
I have generated a short (52 seconds / 8.5 MB) clip showing the prime phases of DNA replication. Please make comments / suggestions as to any improvement. (Not suitable for dial-up lines) --Cookatoo.ergo.ZooM (talk) 08:05, 4 November 2010 (UTC)
File size minimised to 1.11 MB to reduce problems at load and view. --Cookatoo.ergo.ZooM (talk) 20:00, 14 November 2010 (UTC)
I believe the article is good enough to be a Good Article. However not being a major contributor, it is mandatory for regular editors to be consulted prior to submission as I propose. Thoughts? --HarshAJ (Talk)(Contribs) 14:00, 13 December 2012 (UTC)
I noted in section 'Origin' both m-dashes and hyphens were used. It would be best to standardise on n-dashes throughout if hyphens are not appropriate. Style guide gives chapter and verse, and should be read. Sooner or later in the GA/FA process it has to be done, so best to do these things right away.
Likewise, to add refs to unref'd paras, select one or two standard texts and stick to them. I notice you have refs to the out-of-date-edition of The Molecular Biology of the Cell. They should be adjusted to the latest edition, including the new pages numbers.
Boring, but necessary. Macdonald-ross (talk) 12:54, 19 December 2012 (UTC)
Currently (this exact phrasing is a result of my own recent edits, but the article was to this effect before my edits) we say: "The leading strand receives one RNA primer while the lagging strand receives several." Is the later part of this sentence accurate? Are the RNA primers added to the lagging strand as a group of several, or one at a time as the lagging strand is exposed? It is possible that with the initial formation of the pre-replication complex, several RNA primers are added. But since most Okazaki fragments are a few hundred base pairs and the pre-replication complex exposes about the same number of base pairs on the lagging strand, I would imagine only one RNA primer could be added initially. Once replication is proceeding, I would think primers would be added continuously. Thanks!Scientific29 (talk) 18:20, 20 April 2013 (UTC)
Can I ask Miza bot to archive this talk page? The how-to page says get consensus first...Scientific29 (talk) 18:21, 20 April 2013 (UTC)
Merge has been done, but the result is not completely satisfying: I find the article very long and complex and tiring to read, while some points are still not sufficiently explained. For instance I came here looking for details about "stalled replication forks", but the search returns several articles, each of them using the term but without the simplest explanation, and the one place where such an explanation could be expected, precisely "Replication Fork", does indeed mention stalled forks, but with confusing expressions like "If replication forks stall [..] daughter strands have nick obtained un-replicated sites", which are not really helpful.
It would be useful maybe to have a shorter, more readable section "Replication Fork" in this article, with a link to a full main article "Replication Fork", where the subject could be treated really exhaustively, just like it is done in many historical articles in WP.
This way someone could also develop the subsection "Dynamics at the replication fork", which is still somewhat lapidary and rather confusing (what are clamps necessary for? Sentences like Clamp-loading proteins are used to initially load the clamp are not really adding information. And so on), maybe because nobody dares to overload the article even more. 194.174.73.33 (talk) 17:45, 21 March 2016 (UTC) Marco Pagliero Berlin
The comment(s) below were originally left at Talk:DNA replication/Comments, and are posted here for posterity. Following several discussions in past years, these subpages are now deprecated. The comments may be irrelevant or outdated; if so, please feel free to remove this section.
The article is generally scientifically correct, but it badly needs to be translated into plainer English. – ClockworkSoul 04:48, 7 November 2006 (UTC) Changing the rating to "top" as this is high school/SAT biology topic and a central mechanism of molecular biology. - tameeria 03:40, 19 February 2007 (UTC) |
Last edited at 03:40, 19 February 2007 (UTC). Substituted at 12:40, 29 April 2016 (UTC)
Overall, This article is very interesting and the sources are good . Fatin Fatin Hamd (talk) 04:20, 9 September 2018 (UTC)
try to create ribopolymerase in the lab - a catalyst which acts as ribosome and polymerase ... — Preceding unsigned comment added by 2A02:587:4109:4C00:1819:DEC2:D0BC:9571 (talk) 00:14, 29 August 2016 (UTC)
The article makes one reference to the DNA being duplicated (use ctrl-f and search for it to see what I mean) and many references to it being replicated (and is titled as such). Should the single reference to duplication be changed to replication to avoid confusion, or are the terms the same in this context? I'm not well versed on the topic but am confused by the difference and cannot find much online - the article compounds this confusion by mixing the two terms — Preceding unsigned comment added by MattWhitelock0 (talk • contribs) 14:12, 28 April 2018 (UTC)
In the 'dynamics at the replication fork' section topoisomerase and helicase were mixed up (which can be verified by looking at their wikipedia pages, which say that topoisomerase unwinds while helicase cuts). But there is still a thing about DNA gyrase there which is a form of topoisomerase https://www.ncbi.nlm.nih.gov/pubmed/1657531 . And the DNA gyrase page also says that helicase unwinds the DNA. https://en.wikipedia.org/wiki/DNA_gyrase . I don't know enough to confidently edit this so we need someone to fix this. — Preceding unsigned comment added by RotundCat (talk • contribs) 00:07, 24 August 2018 (UTC)
The following Wikimedia Commons file used on this page has been nominated for deletion:
Participate in the deletion discussion at the nomination page. —Community Tech bot (talk) 07:53, 14 May 2019 (UTC)
Last sentence under DNA structure: "The nucleotides on a single strand can therefore be used to reconstruct nucleotides on a newly synthesized partner strand" If the nucleotides on the parent strand are used to construct the partner strand, then the parent strand would be all gone by the time the partner strand was synthesized. No net DNA synthesis would be occurring. To avoid this misunderstanding, say "can be used as a template to direct the order of nucleotides being added to the growing partner strand"? Eaberry (talk) 18:21, 2 June 2021 (UTC)
This article was the subject of a Wiki Education Foundation-supported course assignment, between 21 August 2023 and 15 December 2023. Further details are available on the course page. Student editor(s): Bhavani36 (article contribs).
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