GNAQ, Guanin nukleotid-vezujući protein G(q), podjedinica alfa, je protein koji je kod ljudi kodiran GNAQgenom.[1]
Guanin nukleotid-vezujući proteini su familija heterotrimernih proteina koji se uparuju sa proteinima ćelijske površine, 7-TM domenskim receptorima, i posreduju intracelularnu signalizaciju. Aktivacija receptora katalizuje izmenu GDP za GTP. GDP je vezan za neaktivnu G protein alfa podedinicu. Vezivanje agonista dovodi do konformacione promene i disocijacije kompleksa. G protein alfa i beta-gama podjedinice imaju sposobnost regulacije niza celularnih efektora. Aktivacija se okončava GTPazom specifičnom za G-alfa podjedinicu. G-alfa-q podjedinica je jedan od heterotrimernih GTP-vezujućih proteina koji posreduje stimulaciju fosfolipaze C-beta (MIM 600230).[2]
Za GNAQ je bilo pokazano da interaguje sa natrijum-vodonik antiporterom 3, regulator 1,[3] RIC8A,[4][5]RGS16,[6] beta adrenergički receptor kinazom,[7]RGS4[6][8] i Brutonovom tirozinskom kinazom.[9]
↑Dong Q, Shenker A, Way J, Haddad BR, Lin K, Hughes MR, McBride OW, Spiegel AM, Battey J (Feb 1997). „Molecular cloning of human G alpha q cDNA and chromosomal localization of the G alpha q gene (GNAQ) and a processed pseudogene”. Genomics30 (3): 470–75. DOI:10.1006/geno.1995.1267. PMID8825633.
↑Rochdi, Moulay Driss; Watier Valérie, La Madeleine Carole, Nakata Hiroko, Kozasa Tohru, Parent Jean-Luc (October 2002). „Regulation of GTP-binding protein alpha q (Galpha q) signaling by the ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50)”. J. Biol. Chem. (United States) 277 (43): 40751–9. DOI:10.1074/jbc.M207910200. ISSN0021-9258. PMID12193606.
↑Klattenhoff, Carla; Montecino Martín, Soto Ximena, Guzmán Leonardo, Romo Ximena, García María Angeles, Mellstrom Britt, Naranjo José Ramón, Hinrichs María Victoria, Olate Juan (May 2003). „Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol”. J. Cell. Physiol. (United States) 195 (2): 151–7. DOI:10.1002/jcp.10300. ISSN0021-9541. PMID12652642.
↑Tall, Gregory G; Krumins Andrejs M, Gilman Alfred G (March 2003). „Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor”. J. Biol. Chem. (United States) 278 (10): 8356–62. DOI:10.1074/jbc.M211862200. ISSN0021-9258. PMID12509430.
↑Day, Peter W; Carman Christopher V, Sterne-Marr Rachel, Benovic Jeffrey L, Wedegaertner Philip B (August 2003). „Differential interaction of GRK2 with members of the G alpha q family”. Biochemistry (United States) 42 (30): 9176–84. DOI:10.1021/bi034442. ISSN0006-2960. PMID12885252.
↑Druey, K M; Sullivan B M, Brown D, Fischer E R, Watson N, Blumer K J, Gerfen C R, Scheschonka A, Kehrl J H (July 1998). „Expression of GTPase-deficient Gialpha2 results in translocation of cytoplasmic RGS4 to the plasma membrane”. J. Biol. Chem. (UNITED STATES) 273 (29): 18405–10. DOI:10.1074/jbc.273.29.18405. ISSN0021-9258. PMID9660808.
Raymond JR, Mukhin YV, Gelasco A, et al. (2002). „Multiplicity of mechanisms of serotonin receptor signal transduction.”. Pharmacol. Ther.92 (2-3): 179–212. DOI:10.1016/S0163-7258(01)00169-3. PMID11916537.
Van Oekelen D, Luyten WH, Leysen JE (2003). „5-HT2A and 5-HT2C receptors and their atypical regulation properties.”. Life Sci.72 (22): 2429–49. DOI:10.1016/S0024-3205(03)00141-3. PMID12650852.
Lesch KP, Manji HK (1992). „Signal-transducing G proteins and antidepressant drugs: evidence for modulation of alpha subunit gene expression in rat brain.”. Biol. Psychiatry32 (7): 549–79. DOI:10.1016/0006-3223(92)90070-G. PMID1333286.
Blin N, Yun J, Wess J (1995). „Mapping of single amino acid residues required for selective activation of Gq/11 by the m3 muscarinic acetylcholine receptor.”. J. Biol. Chem.270 (30): 17741–8. DOI:10.1074/jbc.270.30.17741. PMID7629074.
Kabouridis PS, Waters ST, Escobar S, et al. (1995). „Expression of GTP-binding protein alpha subunits in human thymocytes.”. Mol. Cell. Biochem.144 (1): 45–51. DOI:10.1007/BF00926739. PMID7791744.
Allgeier A, Offermanns S, Van Sande J, et al. (1994). „The human thyrotropin receptor activates G-proteins Gs and Gq/11.”. J. Biol. Chem.269 (19): 13733–5. PMID8188646.
Wedegaertner PB, Chu DH, Wilson PT, et al. (1993). „Palmitoylation is required for signaling functions and membrane attachment of Gq alpha and Gs alpha.”. J. Biol. Chem.268 (33): 25001–8. PMID8227063.
Europe-Finner GN, Phaneuf S, Watson SP, López Bernal A (1993). „Identification and expression of G-proteins in human myometrium: up-regulation of G alpha s in pregnancy.”. Endocrinology132 (6): 2484–90. DOI:10.1210/en.132.6.2484. PMID8504751.
Gomeza J, Mary S, Brabet I, et al. (1996). „Coupling of metabotropic glutamate receptors 2 and 4 to G alpha 15, G alpha 16, and chimeric G alpha q/i proteins: characterization of new antagonists.”. Mol. Pharmacol.50 (4): 923–30. PMID8863838.
Petit A, Geoffroy P, Bélisle S (1997). „Expression of angiotensin II type-I receptor and phospholipase C-linked G alpha q/11 protein in the human placenta.”. J. Soc. Gynecol. Investig.3 (6): 316–21. DOI:10.1016/S1071-5576(96)00035-4. PMID8923415.
Kinsella BT, O'Mahony DJ, Fitzgerald GA (1997). „The human thromboxane A2 receptor alpha isoform (TP alpha) functionally couples to the G proteins Gq and G11 in vivo and is activated by the isoprostane 8-epi prostaglandin F2 alpha.”. J. Pharmacol. Exp. Ther.281 (2): 957–64. PMID9152406.
Wise A, Parenti M, Milligan G (1997). „Interaction of the G-protein G11alpha with receptors and phosphoinositidase C: the contribution of G-protein palmitoylation and membrane association.”. FEBS Lett.407 (3): 257–60. DOI:10.1016/S0014-5793(97)00300-1. PMID9175863.