Cellulase (glycosyl hydrolase family 5) | |||||||||
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Identifiers | |||||||||
Symbol | Cellulase | ||||||||
Pfam | PF00150 | ||||||||
Pfam clan | CL0058 | ||||||||
InterPro | IPR001547 | ||||||||
PROSITE | PDOC00565 | ||||||||
SCOP2 | 2exo / SCOPe / SUPFAM | ||||||||
OPM superfamily | 117 | ||||||||
OPM protein | 2osx | ||||||||
CAZy | GH5 | ||||||||
Membranome | 1365 | ||||||||
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In molecular biology, glycoside hydrolase family 5 is a family of glycoside hydrolases EC 3.2.1., which are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]
Glycoside hydrolase family 5 CAZY GH_5 comprises enzymes with several known activities including endoglucanase (EC 3.2.1.4); beta-mannanase (EC 3.2.1.78); exo-1,3-glucanase (EC 3.2.1.58); endo-1,6-glucanase (EC 3.2.1.75); xylanase (EC 3.2.1.8); endoglycoceramidase (EC 3.2.1.123); xanthanase.[8]
The microbial degradation of cellulose and xylans requires several types of enzymes. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family A[9] or as the glycosyl hydrolases family 5.[10] One of the conserved regions in this family contains a conserved glutamic acid residue which is potentially involved[11] in the catalytic mechanism.
In a recent study using Molecular Dynamics simulations, a considerable correlation between thermal stability and structural rigidity of members of family 5 with solved structures has been proved.[12]