Fluorescent dye | Color | mass (g/mol) | Absorb (nm) | Emit (nm) | ε (M−1cm−1) |
---|---|---|---|---|---|
FluoProbes 390 | violet | 343 | 390 | 479 | 24 000 |
FluoProbes 488 | green | 804 | 493 | 519 | 85 000 |
FluoProbes 532 | yellow | 765 | 532 | 553 | 117 000 |
FluoProbes547H | orange | 736 | 557 | 574 | 150 000 |
FluoProbes 594 | red | 1137 | 601 | 627 | 120 000 |
FluoProbes647H | far-red | 761 | 653 | 674 | 250 000 |
FluoProbes 682 | far-red | 853 | 690 | 709 | 140 000 |
FluoProbes 752 | near-IR | 879 | 748 | 772 | 270 000 |
FluoProbes 782 | near-IR | 976 | 783 | 800 | 170 000 |
Abs = absorption maximum#, Em = emission maximum# ..................................[1] ε = molar extinction coefficient |
The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. They are designed for labeling biomolecules, cells, tissues or beads[2] in advanced fluorescent detection techniques.
FluoProbes dye | Color | Light sources (spectral line) |
---|---|---|
FluoProbes 390 | violet | Diode laser |
FluoProbes 488 Fluorescein(FITC)/Cy2 |
cyan | Argon laser (488.0nm), Krypton laser (482.5nm) |
FluoProbes 532 | yellow | Helium–neon laser (632.8nm) |
FluoProbes 547H TRITC/Cy3 |
orange | Argon laser (528.7nm) |
FluoProbes 594 SR101/TR |
red | Argon laser (528.7nm) |
FluoProbes 647H Cy5 |
far red | Krypton laser (647.1nm), Laser (633nm) |
[9] |
Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes.