Guillaume Du Fay (c. 1397–1474) was a French composer and music theorist.

Dufay or du Fay may also refer to:

• Charles François de Cisternay du Fay (1698–1739), French chemist
• Claude Dufay (1926–2001), French entomologist, see Argyrogrammatini
• Jean Dufay (1896–1967), French astronomer
  • Dufay (crater), a crater on the Moon named after Jean Dufay
• Marie-Guite Dufay (born 1949), French politician
• Rick Dufay (born 1952), French-American guitarist, Aerosmith
• DuFay A. Fuller (1852–1924), American businessman and politician

• Dufay River, a tributary of lake Buies, in Québec, Canada

• Dufay Collective, an early-music ensemble from the United Kingdom, specializing in Medieval and Renaissance music and named after Guillaume Dufay
• Dufaycolor, an additive color process for motion pictures

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